When performing the streak for isolation technique, your result is unexpected: There is only growth obtained in quadrant 1. The growth observed in quadrant 1 is as expected, heavy with no isolated colonies. However, no growth is visible in any other quadrants.
Which of the following errors could explain this unexpected result?
A) The loop did not cross into quadrant 1 when streaking quadrant 2
B) You forgot to flame the loop in between quadrants
C) All of the errors listed here could be responsible for this unexpected result
D) Not enough bacteria was obtained from the starting culture when streaking quadrant 1

Answers

Answer 1

Answer:

A) The loop did not cross into quadrant 1 when streaking quadrant 2

Explanation:

The streak for isolation technique is a method used to get isolated bacterial colonies. This technique consists of, in a petri dish with culture medium, where, with the help of an inoculation loop, the bacteria are scratched on one side of the dish. This side is called the first quadrant. This inoculation loop must then cross with the first quadrant and be dragged to the second quadrant, where new lines must be made. This allows a part of the bacteria from the first quadrant to be carried over to the second. The inoculation loop must cross the second quadrant again to inoculate the third quadrant, and so on.

In this case, we can see that for bacterial colonies to grow in all quadrants, it is essential that the loop crosses in the first quadrant to cross out the second quadrant. If not, only the first quadrant will have bacterial colonies.

Answer 2

Answer:

A) The loop did not cross into quadrant 1 when streaking quadrant 2

Explanation:

There are many different techniques to isolate bacteria populations. Using solid media such as agar, you can perform the streak for isolation technique.

What you want to do by using this technique is to get an isolated colony of the bacteria of interest. And to get it, you need to dilute a sample or inoculum of bacteria as much as possible by streaking it. Every time you do it, you take fewer amounts of cells from the original sample, separating them each time more, to the point of having only one single cell apart by a few millimeters from another cell. The isolated cell divides and forms a new pure colony.

So basically, you need to imaginary divide the agar plate into four quadrants.

You sterilize the loop carefully and take a sample of bacteria, trying to get the smallest amount of inoculum. You will apply the loop with the sample in quadrant one and spread it. You close the plate and turn it, placing the second quadrant in front of you. You sterilize the loop again on the flame and let it cool. You perform the same motion in quadrant two but carefully touching the edge of quadrant one, so you can take some of the cells that you have already spread on it. Once you spread quadrant two, you close the plate and turn it again, so quadrant three is in front of you. You sterilize the loop again on the flame and let it cool. You perform the same motion in quadrant three but carefully touching the edge of quadrant two. Close and rotate the plate. You sterilize the loop and repeat the same motion in the last quadrant. Close the plate and take it to incubation. Finally, you put the loop on the flame for the last time.The importance of extending the streaks into the anterior quadrant is to take a few cells from there and to spread them in the following quadrant. In this way, you are diluting the original inoculum, and separating the cells each time more. The fourth quadrant should have a few isolated cells that will reproduce and form a pure colony. If you do not touch the anterior quadrant while streaking, you will not have any cell in the quadrant, and you will not observe any colony after the incubation. The importance of sterilizing the loop every time you change the quadrant is to avoid contamination with other species. If you do not flame the loop, the result will be too many colonies of different species, probably touching themselves, in each quadrant.


Related Questions

If a bacterial isolate show intermediate to moderate resistance to an antimicrobial, how might this drug still be successfully used in the treatment of this microbe

Answers

Answer:

Due to its effectiveness.

Explanation:

This drug still be successfully used in the treatment of this microbe because of its effectiveness on the microbes. All the microbes did not got resistance against the antimicrobial chemical and they are not full proof from the antibiotics so that's why the antimicrobial are used in the treatment of microbes. If all the microbes attain resistance against antimicrobial drug so it can't any affect on the microbes.

A scientist is studying the impact of a new manufacturing plant on the local ocean ecosystem. How could she best monitor the impact of the manufacturing plant on populations in this ecosystem? A. She could perform several wildlife surveys over time to observe any changes in the number and type of marine organisms in the ecosystem. B. She could add a chemical to the water to react with the waste products that the manufacturing plant is dumping into the marine ecosystem. C. She could ask the manufacturing plant to treat all of the waste products that they are dumping into the marine ecosystem. D. She could collect data on the amount of product made by the manufacturing plant and how much the product sells for in local cities.

Answers

Answer:

A. wild life survey

Explanation:

That's was the only answer that involved observing the wild life while the manufacturing plant is operating.

According to Lamarck, the
characteristics of organisms changed
because of:
extinction of competitors
a desire or need for that trait
special acts of creation
genetic mutation
chance

Answers

Answer:

According to Lamarck, organisms altered their behavior in response to environmental change. ... Their changed behavior, in turn, modified their organs, and their offspring inherited those "improved" structures.

trình bày cơ chế hấp thụ và chuyển hóa lipid từ đường tiêu hóa ở người?

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Trong dạ dày được tách rời khỏi các chất thực phẩm khác. Trong ruột non, mật hoa tiết ra chất béo trong khi các enzyme tiêu hoá chúng. Các tế bào ruột hấp thụ chất béo. Các a - xít béo chuỗi dài tạo thành một cấu trúc lipoprotein lớn được gọi là một hạch nguyên tử có thể vận chuyển chất béo qua hệ bạch huyết.

A proteins what can be described as the local folding of a polypeptide

Answers

Here da answer UWU

The next level of protein structure, secondary structure, refers to local folded structures that form within a polypeptide due to interactions between atoms of the backbone

hola como estan soy nueva

Answers

Answer:

Buena gracias

Explanation:

How does water move around the ocean?

Answers

water is constantly moving, but major currents in the ocean are the result of the wind which drags on the surface of the water as it blows.

How many water molecules are needed to break down an oligosaccharide that contains 10 glucose units?
Classify the reaction.

Answers

Answer:

It take ten units of water to break down oligosaccharide that contains 10 glucose units

What 2 things change about the molecules of different states of matter?

Answers

Answer: speed and proximity

Explanation:

science

Explanation:

heating and evaporation

Given that the growing opinion root tip has 16 chromosomes in each cell ( normally). During the cell cycle, while the cell is growing and dividing, specify how many chromosomes will the cell have at the G1 phase? and how many chromosomes will it have after the S phase? Explain your answer with a reason/evidence statement

Answers

Answer:

16

It is given that the number of chromosomes present in each onion root tip cell is 16. Hence, the number of chromosomes in the cell will be 16 at G1 phase, after S phase and after M phase

what elements are creating genetic materials

Answers

Carbon, Oxygen , nitrogen, hydrogen and phosphorus

Can somebody help me?

Answers

Genetic modification
The clone is exactly identical.
Asexual reproduction
Mitosis

Hope this helps, if you need explanations they are below!

Genetic modification changes genes in the plants to make them resistant to the herbicide
The clone is exactly identical because there is only one parent and no sex cells.
The clone undergoes asexual reproduction because it is not sexually mature so it clones to spread
Meitosis is the process of sister cells splitting
This is all based on aloe plants. They produce many clones that can be replanted till sexual maturity when they flower and reproduce with a different aloe

Which of the following is not an accurate description of what science is?

Answers

Answer:

b number is not an accurate description of science

hope it will help

Inang
mukaangaana
wa
men
CARA
mwen
Stible
ther
Macaman
wa
ut​

Answers

Answer:

.........................

Explanation:

.................

Answer:

?????????????????????

what kind of molecule is represented in the diagram?

Answers

Answer:

The answer is D, a nucleotide

Explanation:

Answer:

A carbohydrate or (carb)

Explanation:

Giving me brainiest answer would be awesome!! :D

A cross between a plant with red flowers and a plant with white flowers produces offspring with pink flowers. What is this an example of?

Answers

Answer:

Example of incomplete dominance

Explanation:

the offspring is different the parent

a. The P/O ratio of NADH is the mitochondria is _____________.
b. This means protons are pumped across the mitochondrial membrane by each NADH oxidized starting with complex The P/O ratio of FADH2 in bacteria is_____________.

Answers

Answer:

a). 2.3

b). 1.5

Explanation:

The P/O ratio stands for the[tex]\text{ phosphate / oxygen ratio}[/tex] in the cells. The P/O ratio is defined as the [tex]\text{amount of}[/tex] [tex]ATP[/tex] which is [tex]\text{produced from the movement}[/tex] of the two electrons by a defined electron transport chain and it is terminated by the reduction in the oxygen atom.

The P/O ratio of NADH in mitochondria [tex]10/(3.3 + 1) = 10/4.3 \approx 2.3[/tex]

The estimated P/O ratio for [tex]$FADH_2$[/tex] = 1.5

The complex structures of DNA and protein found in the cell nucleus are mitochondria. nucleoplasm. histones. nucleases. chromosomes.

Answers

Answer:

chromosomes

Explanation:

Genomics refers to the scientific study of genes (DNA) found in living organisms such as humans and animals.

A genome can be defined as the complete set of hereditary instructions that is typically found in the deoxyribonucleic acid (DNA).

The complex structures of deoxyribonucleic acid (DNA) and protein found in the cell nucleus are generally referred to as chromosomes.

In sexual reproduction, the chromosomes from parents are found in the cell nucleus and are comprised of deoxyribonucleic acid (DNA), histone proteins, etc. Thus, they are used to store genetic informations in living organisms.

Basically, the human somatic cell is made up of 46 chromosomes which are sub-divided into 22 pairs of autosomes and a pair of sex chromosomes (X and Y). An autosome is one of the numbered chromosome that is typically not a sex chromosome.

On the other hand, sex chromosomes (X and Y) are responsible for determining the gender or sex of living organisms such as humans.

Assuming the oligopeptide alfahelicks forms one continous a helix the glutamate residue is hydrogen bonded to:___.
a. leucine.
b. cysteine.
c. serine.
d. lysine
e. isoleucine.

Answers

Answer:

B. Glutamate is always bonded to Cysteine when dealing with DNA.

Explanation:

Hope that's what you meant.

If this has to do with RNA or mRNA then still g pairs with c.

Please mark me brainliest!

Shania has a family history of a genetically inherited disease. Based on this information, which statement is true? O Shania has an increased risk of the disease. Shania will get the disease at some point in her lifetime. O If Shania makes unhealthy choices, she will get the disease. The disease will be expressed in all her offspring that have that gene.​

Answers

Answer:

Shania will get the disease at some point in her life

Explanation:

this will happen because the sickness is genetically implanted in her family

Answer:

The diesease will be apart of the genes in her offspring.

Explanation:

Often times mothers with the sickness gene will spread down the gene therefore starting the generational infection in the first place.

Nicholas,, a 3 year-old boy, is brought into your gastroenterology practice by his mother. They were referred to you by their pediatrician because Ali has been complaining of severe abdominal pain, especially after eating, for the past few weeks. He has an otherwise normal health history. His pediatrician ordered a fecal occult (hidden) blood test, hematocrit, and urinalysis. The fecal occult blood test is positive and the hematocrit (red blood cell count) is lower than normal for a child his age. Body temperature and urinalysis are normal.
You perform a surface abdominal exam, which appears normal, but Ali complains of pain in the lower right abdominal quadrant during palpitation. You order a CT enterography procedure, which uses a contrast dye to make an X-ray of the small intestine. The CT shows a diverticulum. After confirmation with a technetium scan, you diagnose Nicholas withMeckel’s diverticulum.

Answers

Treatment for Meckel’s diverticulum includes surgical removal of the diverticulum.

What do you mean by diverticulum?

Diverticulum is a small pouch or sac that forms in the wall of the digestive tract. It can occur in any part of the digestive tract, including the esophagus, stomach, small intestine, and large intestine. Diverticula can cause symptoms such as abdominal pain, constipation, and diarrhea.

You recommend that Nicholas have a laparoscopic removal of the diverticulum, a minimally invasive procedure that involves making small incisions in the abdomen and inserting a camera and surgical tools to remove the diverticulum. You explain to Nicholas’ mother the benefits of the procedure and the potential risks associated with it.

After the procedure, you recommend that Nicholas follow up with his pediatrician in two weeks. You also suggest that Nicholas and his mother modify his diet to include more fiber and fluids to help ease his symptoms and prevent the diverticulum from returning. You also suggest that Nicholas take an antacid to help reduce the abdominal pain.

To know more about diverticulum,

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CODIS is divided in to Indexes. (Offenders, Forensic Unknowns, and Relatives of Missing Persons etc.) Is law enforcement allowed to search all of the profiles in all indexes against each other? Why or why not?
Question 2 options:

1)

Yes, if the data is in the database then it is lawful to search it anyway that helps the case.

2)

No, but only because it is not helpful to the case.

3)

Yes; but only if you have the permission of the victim in the case.

4)

No; it is against a federal law.

5)

Yes; but only in cases when the suspect is a male.

Answers

Answer:

1

Explanation:

they are always allowed to

the colour of chameleon is not it beauty but for what purpose​

Answers

The reason chameleons change color is to allow them to camouflage where ever they are so that they can hide better from predators.

Choose All that apply !!
Help!!

Answers

Answer:

1.5

Explanation:

The greatest probability must not exceed 1

[tex] { \boxed{ \sum(P) \leqslant 1}}[/tex]

Give three reasons why you might have a situation in which bacterial colonies were found on the first section of a streak plate, but not on sections two and three.

Answers

Hi. You have not shown the sections this question refers to, nor have you provided more information about those sections. This makes it difficult for your question to be answered. However, I will try to help you as best I can.

Generally speaking, we can consider that there was no growth of bacterial colonies in sections 2 and 3, because, for some reason, the bacteria was not inoculated in sections 2 and 3. What could also have happened, is that the bacteria in the sections 2 and 3 were eliminated, but the bacteria from section 1 managed to survive and form colonies.

Another possibility is that after inoculating the bacteria in section 1, you didn't handle the bacteria correctly in the other sections, leaving that bacteria to dry out and die.

Finally, the culture media in sections 2 and 3 could be inappropriate for bacteria to develop and form colonies.

When proteins help transport molecules in and out of the cell, it is called _____.

passive diffusion
facilitated diffusion
active transport
endocytosis and exocytosis

Answers

Answer:answer is active transport

Explanation:

What is transaction between school and post school destination​

Answers

Answer:

The transition between school to a post school is a difficult phase in one's life because it opens a lot of adjustments whether it is socially, mentally and/or psychologically. Most students find it difficult to transfer because of these following reasons visit these links and see the answers below: brainly.ph/question/2641856, brainly.ph/question/2635171, brainly.ph/question/2627976

There are a bunch of strangers. Not all students are gifted with an extropersonal skills that allows them to make friends easily. Transition makes it difficult for introvert pupils to accept.

The new school is bigger than their previous school. Though in others the size of a place is not bothering their performance, there are individuals who find it difficult to adjust when it comes to the size of their environment. For example a student who studied in a small private school with only one section will find it difficult to adjust moving around a larger environment.

There are different teaching styles coming from the school's faculty. Learning styles are somewhat define by the teacher's teaching styles and strategies. In their previous school, some of the seniors might have already leaked the strategies used by the teacher in their school. But in a post school, some students are 100% newbie wherein they have no idea what is going on inside a class of a single professor or instructor. This is difficult since they do have to observe every single day and learn a new way to follow the lesson's phase.

Friends are not with the same post school. It is said that you can go through anything with your friends but most of the time you will never be with them when you transfer to a post school but still you need to move forward and learn to mix with the public.

Lessons are difficult and new. Most learners are not accustomed in learning quick in a new environment especially when the lesson is more difficult and new.

New policies are too intimidating to break. Some students are struggling when it comes to adjusting to rules whether it is inside the classroom or just a simple campus rule so they feel intimidated and and fearful in breaking one. But it must be remembered that rules keep the society moving forward peacefully.

Explanation:

There are different reason for an individual to fear and feel difficulty adjusting to a post school. But one must remember that humans are created with an adaptability skill that allows us to mix well with a new habitat and environment. Social skills are just part of our adaptability. You can just follow the wind and let the flow take you. Life is a constant change and all you have to do is to embrace it to make yourself belong anywhere you go.

Transaction between school and post school destination​ is the entering from one stage to another stage of live.

What is transaction between school and post school destination​?

The transition between school and post school destination means that the youth is ready for next stage of their lives. The perfect transition of the students from high school to college or the adult world is very important.

This is because these years provides the direction to the students for their future so we can conclude that transaction between school and post school destination​ is the entering from one stage to another stage of live.

Learn more about transaction here: https://brainly.com/question/24835236

What part of the cell does: Photosynthesis? Respiration?

Answers

Photosynthesis occurs in the chloroplasts, whereas cellular respiration occurs in the mitochondria. Photosynthesis makes glucose and oxygen, which are then used as the starting products for cellular respiration.



I hope it helps you this! :)

A group of organ systems makes up a

Answers

Answer:

A group of organ systems name up the cardiovascular system.

organ systems make up your natural bodily function

10 POINTSSSSSSSSS!!!!!!!!!!!!!!!!!!

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Answer:

13. A group of Roaches moving in a group

14. Stepping on the Roaches to kill them

Answer: First image shows 3 groups with 14 Roaches in each group moving towards an human.

Second image shows the human stepping on the middle group of roaches.

Explanation: Roaches are nasty. Get an exterminator, ya nasty.

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