Fluorescent FM dyes partition reversibly into biological membranes without penetrating through them. Suppose that you have neurons cultured in a dish, and you incubate them with an FM dye for a period of time before washing the cells with excess medium to remove the dye.a)You find that if you stimulate the neurons to trigger action potentials during incubation with the dye, the synaptic regions of the neurons remain fluorescent after the wash. Propose an explanation for this effect.b)After you have generated fluorescent synaptic regions by this procedure, suppose that you trigger further action potentials while continuing to wash with excess medium. Would you expect the synaptic regions to lose fluorescence

Answers

Answer 1

Answer:

a) Emission observed is due to emission of synaptic vessels

b) Yes

Explanation:

a) Proposing an explanation

The emission observed ( the synaptic regions of the neurons remaining fluorescent ) is due to the emission on the synaptic vessels given that FM dyes have lipid-sensitive emission which can be loaded into a recycled synaptic vesicle

b)

The synaptic regions will lose fluorescence when you continue to wash with excess medium, also the more action potentials triggered the more observed emission. hence the increase in the loss of fluorescence is due to the fact that the emissions are not permanent


Related Questions

please I really need a help here ASAP. It's a physics question.

A space ship travels 3.00 ~ 10 km vertically to transport scientists from one particular station to
another. After the transportation, the captain decides to go hizontally 2.00 * 10km to visit an Orie
Calculate its:
a. Distance
b. Displacement​

Answers

Letter B, displacement

Answer:

a).Don't know if you meant to write 3x10^10km and 2x10^10km

I'll solve with this... If they aren't the values... You can then input the real values.

Distance = Total length traveled.

D= 3x10^10 + 2x10^10

D= 5x10^10Km

b) Displacement:

Since it went vertically... Then went horizontally in the second phase... It is forming a right angled Triangle and the Displacement would be the Hypotenuse of this triangle.

Disp.= √ (3x10^10)²+(2x10^10)²

Disp.=3.61x10^10km.

BRAINLIEST
CAN SOMEONE PLZ HELP ME!!

Answers

Explanation:

I’m pretty sure it’s phenotypes

Please do not answer if you don't know this is a final I really need to pass 

Answers

D
A

i learned this last year

also pls give brainliest

(I really need this to boost my grade)

Label the permeable and impermeable rock layers

Answers

Answer: HI your question is incomplete but i will provide a general answer that can help you

answer : Permeable Rock layers allow the flow of fluid like substances through them, While Impermeable rock layers do not allow the flow of fluid like substance through them.

Explanation:

Permeable rocks are rocks containing pores through Fluid like substances can penetrate through in the rocks. examples of Permeable rocks are ; Sandstones and Chalk

While an Impermeable rock is a rock that does not allow the flow of fluid like substance through it due to the absence of pores in its rock layers . examples of such rock are ; Clay and marble  

Although some permeable rocks might exhibit low level of permeability as well but generally Rock sample with pores are classified as permeable rock layers.

Advantages and disadvantages of physical barriers in the immune system

Answers

Explanation:

advantages:Physical barriers play an important role in preventing microbes from reaching tissues that are susceptible to infection. At the cellular level, barriers consist of cells that are tightly joined to prevent invaders from crossing through to deeper tissue.

Physical barriers play an important role in preventing microbes from reaching tissues that are susceptible to infection. At the cellular level, barriers consist of cells that are tightly joined to prevent invaders from crossing through to deeper tissue.

What is immune system?

Immune system is a large network of organs, white blood cells, proteins (antibodies) and chemicals. This system works together to protect you from foreign invaders (bacteria, viruses, parasites, and fungi) that cause infection, illness and disease.

Disadvantages of physical barriers in the immune system is causing immuno deficiency disease.

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the soil will move farther when you pour water_________

gradually or rapidly

Answers

Answer:

The soil will move farther when you pour water gradually.

Scientists observed that the populations of top-level consumers in a particular ecosystem were rapidly decreasing. Further studies revealed that there was also a decline in producer productivity. Which other changes did the scientists most likely observe in the ecosystem?


increased producer diversity


decreased population size at all levels


decreased primary consumer populations only


increased primary and secondary consumer diversity

Answers

Answer:

The correct answer is - decreased population size at all levels.

Explanation:

A decrease in the productivity of producers of an ecosystem will lead to a decrease in all other organisms of the ecosystem including top-level consumers to the bottom-level producers due to the fact that producers provide energy and food to primary consumers.

It is because they are the only organisms that convert the sunlight to usable energy and food that transfer from one trophic level to other, so if the population of producers or their productivity will decrease then there will be decreased population size at all levels.

What are two things we need to consider to make living off Earth possible?​

Answers

Oxygen and Food? Tell me if that ain’t it :)

ells go through periods of growth and division. Cell division occurs during

Answers

Mitosis is when the cell divides

Rheumatoid arthritis is commonly associated with the presence of rheumatoid factor autoantibodies in the bloodstream. This indicates that rheumatoid arthritis is likely to be a. an infective process. b. caused by bacterial infection. c. an autoimmune process. d. because of an enzymatic defect.

Answers

Answer:

The correct answer is - c. an autoimmune process

Explanation:

Rheumatoid arthritis is a disease or condition that affects the joints mainly by swollen, and painful joints. It is an autoimmune disease as the person's own immune system attacks the body.

The presence of rheumatoid factor autoantibodies states that it is an autoimmune process. There are other autoantibodies also associated with this type of arthritis that are rheumatoid factors (RF), anti‐keratin antibodies (AKA), anti‐filaggrin antibodies (AFA), and anti‐perinuclear factor (APF).

Why does climate change occur?
Explain your answer.''

Answers

Answer:

Human activity is the main cause of climate change. People burn fossil fuels and convert land from forests to agriculture. Since the beginning of the Industrial Revolution, people have burned more and more fossil fuels and changed vast areas of land from forests to farmland.

Explanation:

A fruit fly of genotype B R/b r is testcrossed with b r/b r. In 84 percent of the meioses, there are no chiasmata between the linked genes; in 16 percent of the meioses, there is one chiasma between the genes. What proportion of the progeny will be B r/b r

Answers

Answer:

4 percent (4%)

Explanation:

A single crossover occurs between two (non-sister) chromatids belonging to homologous chromosomes. In this case, 16 percent of the meioses have a single crossover, thereby it will produce 8 percent of the chromosomes with the original (parental) combination in the progeny and the remaining 8 percent should be recombinants. From this result, it is reasonable to conclude that half of these recombinants should be 'Br' (and the other remaining 4 percent should be recombinants 'bR'), and therefore the answer is 4 percent (4%).

What is the answer to number 1

Answers

Answer:

TTT GGC AGT

Explanation:

Which of the following would not produce more electricity when a magnet is moved
back and forth through a coil of wire?
1 moving the magnet faster
2 adding more coils
3 using a stronger magnet
4 using longer wire​

Answers

Answer:

moving the magnet faster thats the answer i can give not sure bit by incerasing coils electrycity is more produced

Moving the magnet faster  would not produce more electricity when a magnet is moved.

What is magnet?

A substance or thing that creates a magnetic field is called a magnet. The most noteworthy characteristic of a magnet—a force that pulls on other ferromagnetic elements, such as iron, steel, nickel, cobalt, etc.—as well as the ability to attract or repel other magnets—is caused by this invisible magnetic field.

An object made of a substance that has been magnetized and produces its own persistent magnetic field is referred to as a permanent magnet. A common example is a refrigerator magnet used to keep notes on the door of the refrigerator.

Ferromagnetic materials are those that can be magnetized and are also those that are powerfully drawn to a magnet (or ferrimagnetic). These comprise some naturally occurring materials as well as the metals iron, nickel, and cobalt as well as their alloys.

Therefore, Moving the magnet faster  would not produce more electricity when a magnet is moved.

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female male or other

Answers

Female because yea….!;)4!:973)?485(4$93?
Are- are we guessing yours or our own?

Certain species of blind fish living in perpetual darkness in the ocean depths have evolved from ancestors that could see. Research indicates that the brain centers for vision in these fish is degenerating, whereas the brain centers controlling other senses are enlarging. What might be the MOST probable reason for the loss of vision in these fish

Answers

Answer:

Explanation:

Evolution for lack of light. If there is no reason for it you lose the ability but it strengthens other senses. It's compensation. The need to tell if the temperature has increased because of something nearby or ability to feel miniscule movement will increase to compensate for that lack of sense.

Artificial wetlands ________. are the major program for replacing lost natural wetlands purify water for use as bottled drinking water are a source of arsenic contamination in Bangladesh are created using xeriscaping methods can help purify water and also provide wildlife habitat

Answers

Artificial wetlands can help purify water and also provide recreational opportunities. They are the major program for replacing lost natural wetlands purify water for use as bottled drinking water are a source of arsenic contamination in Bangladesh are created using xeriscaping methods can help purify water and also provide wildlife habitat.

Please answer the questions

Answers

No yendo ni idea de lovqu es

this is due in 50 minutes

Answers

Answer:

It should have taken over 2500 million years. This is owing to many natural disasters as well as the rotation of the planet, which resulted in the separation of landscapes.

Explanation:

True or False; Carbohydrates and Lipids are organic, contain CHO and are both forms of energy.

Answers

The answer is true! :) hope it helped

PLEASE ANSWER ASAP I AM BEING TIMED!!!
ONLY ANSWER IF UR 100%
EXPLAIN THIS IS A WRITTEN ANSWER

Briefly explain why fossil fuels are good sources of fuel. In other words, where does the energy come from?

Answers


Fuel economy improvements: save you money, reduce climate change, reduce oil dependence costs; and increase energy sustainability.

What is microbiology about?

Answers

Answer:

Microbiology is the study of all living organisms that are too small to be visible with the naked eye. This includes bacteria, archaea, viruses, fungi, prions, protozoa and algae, collectively known as 'microbes'.

Explanation:

it right here -w-

If the amount of food or resources available increases, the carrying capacity of the animal would ?

Answers

Answer: Increase

Explanation:

Increase




Explanation: did this in middle school

WILL MARK BRAINLIEST!!!
Which of these is a benefit for a parent who sees a genetic counselor?

A. Deciding whether or not to take medications before pregnancy.
B. Being able to plan in advance for a genetic disorder in a child.
C. Knowing that society must make decisions about genetic disorders.
D. Having a source of long-term psychological help to live with a genetic disorder.

Answers

Answer:

B. Being able to plan in advance for a genetic disorder in a child.

Being able to plan in advance for a genetic disorder in a child is an advantage for a parent who sees a genetic counselor. So, the correct option is (B).

What is Genetic Disorder?

A genetic disorder is a type of disease caused in whole or in part by a change in a DNA sequence away from the normal sequence.

It can be caused by several reasons like

1. Monogenic Disorder

It is caused by a mutation in one gene

2. Multifactorial Inheritance Disorder

It is caused by mutations in multiple genes

3. Combination of gene mutations and environmental factors.

4. Damage to chromosomes by the changes in the number or structure of entire chromosomes, the structures that carry genes.

Genetic counseling helps parents for better understand test results and treatment options, helps deal with emotional concerns, and provides referrals to other healthcare providers and advocacy and support groups.

Thus, being able to plan in advance for a genetic disorder in a child is an advantage for a parent who sees a genetic counselor. So, the correct option is (B).

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Scientists have determined that Earth's interior has layers with different properties. One property is that the inner core is solid. Which of the following statements can be used as evidence to support this property?
A.
During an earthquake, both P waves and S waves travel away from the focus through the interior of Earth.
B.
After an earthquake, P waves are detected on the opposite side of Earth but S waves are not.
C.
P waves do not refract based on the density of the medium it is traveling in, but S waves do.
D.
Seismographs that are far from the epicenter of an earthquake detect P waves much sooner than S waves.

Answers

Explanation:

During

an earth quake both p waves and s waves travel away from the focus

Answer:

B

Explanation:

B


The diagram shows three steps in the carbon cycle.
The Carbon Cycle

Which two phrases best summarize the events taking place in Step 2?

A. Cellular respiration

B. Storing carbon dioxide in living organisms

C. Photosynthesis

D. Releasing carbon dioxide into the atmosphere

Answers

Answer:

A. Cellular respiration

C. Photosynthesis

Explanation:

There are two main processes occurs in the carbon cycle i.e. cellular respiration and photosynthesis. Plants absorb carbondioxide gas from the atmosphere through small openings called stomata. The carbondioxide enters the leaves and combine with water produces glucose and oxygen. Glucose is stored in different parts of plant whereas some oxygen is used by the plant and the remaining oxygen is releases in the atmosphere while on the other hand, in cellular respiration, glucose is broken down to produce energy in the form of ATP, carbondioxide gas and water.

Answer:

The real answer is A and D.

What is the kinetic theory of matter? How does it relate to the motion of molecules?​

Answers

Explanation:

kinetic energy is energy that object has because of its motion .The kinetic molecules theory explain the forces between molecules and the energy that they possess. matter is composed of small particle's(atom,molecules,and icon)

What is the major source of energy for the Earth?

Answers

here's your answer.. Option (C): the sun

What are the answers for both of the questions

Answers

the answer would be CCC AGU CGA GUC UAU
the amino acids would be: Pro, Ser, Arg, Val,and Tyr

In screenshot! answer quickly please

Answers

Answer:

umm i would answer but i cant see the photo whats the question?

Explanation:

Other Questions
Hart Attorney at Law experienced the follwoing transactions in 2016, the first year of operations:1. Accepted $36,000 on 4/1/16, as a retainer for services to be performed evenly over the next 12 months (2) Performed legal services for cash of $54,000 (3) Purchased $2,800 of office suppies on account (4) Paid $2,400 of the amount due on accounts payable (5) Paid a cahs dividend to the stockholders of $5,000 (6) Paid cash for operationg expenses of $31,000 (7) Determined that at the end of the accounting period $200 of office supplies remained on hand (8) On 12/31/16, recognized the revenue that had been earned for services performed in accordance with Transaction 1Problem: Show the effects of the events on the fianncial statements using a horizontal statement model. Phyllis Schlafly a ran for President in 1972 b created the Special Olympics c considered the defeat of the ERA as a victory for women's rights. d fought diligently for the passage of the ERA. I need help!!!!!!!!!!!!!!!!!!!!!!!!!!!!! PLEASE HELP. I NEED ASAP WILL GIVE BRAINLIEST. WORTH A LOT OF POINTSRonald was given the pair of equations shown below, which are in slope-intercept form (y = mx + b):y = 3x 2y = 5x 6Part A: Ronald does not know how to solve the system of equations. Help him by writing the slope (m) and y-intercept (b) for each equation, and in your own words, explain how you can solve the pair of equations graphically.Part B: Now that you have helped get Ronald started, solve the system and write the solution to the pair of equations in (x, y) format so Ronald can check his work and answer. What is the main problem with this introduction paragraph? We're your parents wishing for a boy or a girl when they were expecting you? Many forms of recreation have been unfairly classified as for boys or for girls This is a shame because it makes both boys and girls afraid to try activities that they could really enjoy. Boys and girls should be allowed to participate in any activities they enjoy and should be supported by friends and family in doing so. (BTW THIS IS LANGUAGE ARTS LOL) What is taxes? What is Texas' tax rate? Simplify for ez points! Expand and combine like terms.(4b^2+ 3) (4b^2- 3) =1SEE ANSWER PLZ HELP PLZ PLZ ILL MARK AS BRAINLIESTT!! based on the graph below, which statement Sandhill Co. purchased machinery that cost $2800000 on January 4, 2019. The entire cost was recorded as an expense. The machinery has a 9-year life and a $180000 residual value. The error was discovered on December 20, 2021. Ignore income tax considerations.Before the correction was made, and before the books were closed on December 31, 2013, retained earnings was understated by:____. If the U.S. engages in free trade and the international price of skateboards is $75, it would import _____ skateboards from the rest of the world. Is this an alpha or beta decay? Which country is not the origin of Latinos? Spain Guatemala Chile Brazil Solve for x algebraically x/4 6 Assignment: Provide a reflection paper of 500 words minimum (2 pages double spaced) of how the knowledge, skills, or theories of Risk Management and Information Security have been applied, or could be applied, in a practical manner to your current work environment. If you are not currently working, share times when you have or could observe these theories and knowledge could be applied to an employment opportunity in your field of study.Requirements: Provide a 500 word (or 2 pages double spaced) minimum reflection. Use of proper APA formatting and citations. If supporting evidence from outside resources is used those must be properly cited. Share a personal connection that identifies specific knowledge and theories from this course. Demonstrate a connection to your current work environment. If you are not employed, demonstrate a connection to your desired work environment. You should NOT provide an overview of the assignments assigned in the course. The assignment asks that you reflect how the knowledge and skills obtained through meeting course objectives were applied or could be applied in the workplace. Which of the following is not a nonmetal: sulfur, oxygen, silicon, or nitrogen? What happens when magma moves the tectonic plates and pressure builds up The coordinates of the vertices of BEA are B(1,2), E(-5,3), and A(-6,-3). Prove that BEA is isosceles. State the coordinates of point R such that quadrilateral BEAR is a square. Prove that your quadrilateral BEAR is a square. 150 round 2 decimal places