During the bacterial transformation lab, what was the primary purpose of including Ampicillin in the agar

Answers

Answer 1

Answer:

allows for selection of E. coli mutants that successfully picked up the plasmid

Explanation:

Ampicillin is a penicilin type medicine or antibiotic that can treat the infections caused by bacteria.

In bacterial transformation lab, the ampicillin is added in the agar in order to select the E.coli mutants which picked the plasmid and have gained ampicillin resistance gene. Bu doing so, it causes the production of the enzymes that prevents the activity of the ampicillin as well as the bacteria survive. The ampicillin antibiotics present in the agar kills the bacteria without having the ampicillin resistance gene.


Related Questions

GORAN
NARGO MYSETS
OMSRANOG
LECL
SUSTIE
Unscramble these words

Answers

I just answered your other post with these questions. (:

WHAT TYPE OF MUTATION IS THIS?

Answers

Answer:

Missense mutation. This type of mutation is a change in one DNA base pair that results in the substitution of one amino acid for another in the protein made by a gene. Nonsense mutation. A nonsense mutation is also a change in one DNA base pair

Explanation:

which of the following is not a constant?

a. plant height
b. types of beans
c. amount of fertilizer
d. hours of sun exposure

Answers

Answer:

a. plant height.

Explanation:

As you can see in the diagram the plant heights are varied.

Do you believe that all of our actions can be attributed to the nervous system? Does your memory of an event trigger your responses? Why or why not?

Answers

Answer:

The nervous system is made up of all the nerve cells in your body. It is through the nervous system that we communicate with the outside world and, at the same time, many mechanisms inside our body are controlled. The nervous system takes in information through our senses, processes the information and triggers reactions, such as making your muscles move or causing you to feel pain. For example, if you touch a hot plate, you reflexively pull back your hand and your nerves simultaneously send pain signals to your brain. Metabolic processes are also controlled by the nervous system.

Explanation:

Grade 8 2020-2021
Which form of energy is almost always produced during energy transformations?
O
A heat
B. electricity
C. light
D. sound

Answers

Answer:

A

Explanation:

Because I searched it up

Answer:

A

Explanation:

During transformations from one form to another, heat energy is almost always produced. Whenever a form of energy is converted to the other form, heat is almost always released during the reaction.

out of all these animals which is the biggest emu kangaroo kookaburra wombat

Answers

imma say an emu. emus are pretty scary too ngl

Answer:

kangaroo

Explanation:

HELP PLSSSS
Question 24
2 pts
What is the difference between a question and a hypothesis?
A question is an assumption and the hypothesis is the answer to the assumption.
A question is developed from some observations and the hypothesis is a tentative explanation for that
question.
A question is what you end up with after the test and the hypothesis is a summary of the conclusions.
A question is the summary of data collected and the hypothesis is the interpretation of the data
Question 25
2 pts.

Answers

Answer:

A question is developed from some observations and the hypothesis is a tentative explanation for that

Explanation:

How do human sex chromosomes keep the numbers of males and females roughly equal?

Answers

Answer:

It is simple mathematics , because sex chromosome XX are found in female and XY in male the remaining 22 pairs are the same in both.

Explanation:

I looked it up. I'm sorry if it's wrong.

Mature age woman learners list ___ as the most common reason for not completing their degress

Answers

Answer:

Family is the answer

Explanation:

Mature woman have a lot of responsibilities on there shoulder, th house, the kids, the budget planning, job work etc. They just can't have a single minute to waste upon, they have to handle so many things, and they do that all with single hand. They make things perfect and that takes a lot of their time, after doing this all daily chores even Jesus wouldn't have energy to do anything, so how do you expect any human. Woman plays a very responsible role from beginning of the day to night.

11
Sea otters move into the ocean bay. The eat all the sea urchins. This change will cause the
A. Kelp to have less food
B. crabs to have less food
C. Sea ducks to have more food
D.Arctic foxes to have more food

Answers

well sea urchins are primarily food for crabs, so by sea otters eating the sea urchins crabs will end up with no good

The presence of sea otters in an ocean bay will result in a reduction in the amount of food available to carbs because they feed on sea urchins. Therefore, option (B) is correct.

What is food chain?

The linear relationship between creatures that demonstrates how they obtain energy by feeding on one another is referred to as the food chain.

The information provided in this inquiry suggests that sea otters migrate into the ocean bay and consume all of the sea urchins there.

This leads one to believe that they cut down on the food supply of another eater of urchins, such as carbs. Because of this, the introduction of sea otters into the ocean bay will result in there being less food available for carbs.

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Select the part whose main job is to direct a plant cell's activities by sending instructions to
different parts of the cell.

vacuole

chloroplasts

mitochondria

nucleus

Please hurry I’m in a rush

Answers

I think it’s the nucleus

Nucleus part main job is to direct a plant cell's activities by sending instructions to different parts of the cell.

What is the short definition of a plant cell?

Plant cells are eukaryotic cells with a genuine nucleus and particular organelles to carry out particular tasks. Plant cells do, however, include several organelles that are unique from those seen in other eukaryotic cells.

Where can you find plant cells?

Roots, stems, leaves, flowers, and reproductive structures are only a few of the major classes of cells and tissues that are formed when plant cells differentiate from undifferentiated meristematic cells, which are similar to the stem cells of animals.

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How do plant cells differ from animal cells

Answers

Answer: Plant cells have chlorophyll, chloroplasts, cell walls, a more blocky shape, and larger vacuoles. Animal cells have a more rounder shape, no cell wall but only a cell membrane, smaller vacuoles, and other organelles such has centrosomes, which plant cells don’t have.

Explanation:

Answer:

Plant and animal cells are  different in that plant cells have chloroplasts and rigid cell walls, while animal cells do not.

what are the cells that divide to produce gametes ?

Answers

Answer:

cells needed for sexual reproduction

Explanation:

so that during fertilization,the total number of chromosomes will be 46..

What are the cells that divide to produce gametes? germ cells

What is a gamete?

It is the reproductive cell of an organism. They are haploid in nature. 

How many types of gametes are there?

There is a male gamete that is termed sperm (animals) or pollen grain (plants) and a female gamete that is termed ovum (animals) or egg (plants).

What kind of division occurs prior to gamete formation?

Meiosis, or reductional division, occurs prior to gamete formation.

What is its significance?

It ensures that after fertilization, the normal diploid number is maintained.

What kinds of cells undergo this division to produce gametes?

Germ cells are the cells that produce gametes. 

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At which type of boundary do rocks react to tectonic stress and pull away leading to the formation of new crust?

Answers

Answer:

Divergent boundary.

Explanation:

At divergent plate boundaries, plates react to tectonic stress and move away from each other. Magma from the mantle hardens on the edge of each plate, forming new crust. I hope this helps!

Woodpeckers peck trees to find food and make nests. What would happen if
most of the trees are cut down?

Answers

Answer:

the wood packers wouldnt have a home to live in anymore. the effect of this means that the habitat for them and other animals is destroyed and they could start to die and become endangered species. this is my opinion

Explanation:

This is from what I have learned so far

Similar patterns of embryological development in different but related organisms are most likely due to similarities in their
environment
analogous structures
ancestory
fossil forms

Answers

Answer:

fsaf

Explanation:

dsdadsda

Complete each statement with the correct term from the drop-down menu.

Answers

Explanation:

but where is statement to correct it ?

Answer:

1. Carbohydrate moves molecules

2. Lipids provide structure for plants

3. Carbohydrates determine what people look like

4. Cells are surrounded and protected by Carbohydrates

5. Proteins speeds up chemical reactions in the body

6. Hemoglobin is built by Nucleic Acids.

7. Human body is insulated by lipids

Explanation:

How does the presence of suspense in a story affect readers?

It helps them predict the story’s outcome.
It gives them hints about the characters’ thoughts.
It makes them skip the boring parts.
It keeps them engaged in the story.

Answers

Answer:

Suspense ensures the reader will have enough interest to continue reading throughout the piece. If the author has done his job, suspense will continue to increase up until the climax, or the final confrontation and turning point.

Explanation:

Hope this works out my friend

It keeps them engaged in the story. So, the correct option is (D).

What is Suspense?

Suspense is defined as a state of mental uncertainty, worry, being undecided, or being doubtful. It is the anticipation of the outcome of a plot or the resolution of an uncertainty, riddle, or mystery, especially as it affects a character for whom there is sympathy.

Following are the types of suspense :

Narrative SuspenseShort-term SuspenseRomantic/Comedic SuspenseCliffhangersMysteryHorror

The smart use of suspense in a story that helps build suspense and keep the plot engaging as the reader progresses, and sets the stage for genuine surprise and shock when a major plot point or twist is revealed from an unexpected angle.

Thus, it keeps them engaged in the story. So, the correct option is (D).

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1) Jupiter, mars, and_______ have wind
2) ______ means to wear away
3) at a wind farm they harvest_______ energy

Answers

Answer:

1)Earth

2)disappear

3)Solar energy

Explanation:

I am not sure but hope it helps let me know if its correct.

GOOD LUCK!

In science fiction stories, plants that live on planets far from the sun are often described as having black foliage, while those that live on planets close to the sun are described as having shiny reflective foliage. If the plants on these planets do photosynthesis similar to terrestrial plants, discuss why these colors would be good adaptive features.

Answers

Answer:

See the answer below

Explanation:

The black foliage of the plants that live on planets far from the sun is an adaptive feature to maximize the heat from the sun for their metabolic activities. A certain level of temperature is usually required for optimal metabolic activities and the distance away from the sun means that heat could be a limiting factor. A black color, however, is able to absorb all the available heat in the surrounding. Hence, the black leaf color serves to maximize the heat from the sun for the benefits of the plant.

The shiny reflective foliage of the plants that live on the planets close to the sun can also be viewed as an adaptive feature to minimize the effects of the proximity of the sun. Too much sunlight will cause the tissues of the leaf to heat up beyond the optimal temperature requirement for metabolic activities. A shiny reflective color will reflect more of the rays of light from the sun than it absorbs. Thus, the foliage is prevented from overheating.

Living cells cannot use heat to provide the activation energy for biochemical reactions because

Answers

they use ATP and ADP?

What event in 1927 was a main reason that the U.S. Army Corps of Engineers implemented many projects in the following years to better protect people and property in the Mississippi delta?
A. an unforeseen dramatic increase in the number of migrating birds passing through Louisiana
B. a major that affected the entire lower Mississippi River valley
C. major hurricane that destroyed most of the buildings in New Orleans and the surrounding areas
D. major oil spill in the Gulf of Mexico areas

Answers

Answer:

B

Explanation:

Answer:

b

Explanation:

what is the role of the grana in chloroplast?

Answers

Answer:

Grana are the structures formed of coin like structures called thyllakoids that encloses chlorophyll for absorbtion of light essential for photosynthesis.And grana also give green colour to chloroplast.

Explanation:

HELP PLZZZ!!!......Which feature is created by wave erosion?

A)loess
B)delta
C)rill
D)stack

Answers

Answer:

the answer is d stack

Explanation:

A feature which is created by wave erosion is Stack. Thus, the correct option is D.

What is Wave erosion?

Wave erosion can be defined as the waves which are creating a variety of landforms along with a shoreline. Sea cliffs generally occur when the waves erode rock, resulting in the high slopes formation. Wave energy results into production of erosional formations such as cliffs, wave cut platforms, sea arches, and the sea stacks. These all are features of wave erosion.

Sea wave erosion is accomplished primarily by the hydraulic pressure, the impact of waves that are striking the seashore, and through the abrasion that is wearing, grinding, or rubbing away by the friction by sand and pebbles that are agitated incessantly by the water and wave-cut platform.

Therefore, the correct option is D.

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Which characteristic of life distinguishes a fire and a flowering plant?

Answers

Answer:

crytaExplanation:

Answer:

The use of energy is a life characteristic that distinguishes fire from a flowering plant

Explanation:

When fire is put on, it makes use of oxygen and releases carbon dioxide and other gases. Burning of fire makes use of heat energy

What does geological time have to do with rocks?

Answers

Answer: Measuring the amounts of radioactive elements in rocks let scientists useabsolute dating to give ages to each chunk of time on the geologic time scale. For example, they are now able to state that the Jurassic began about 200 million years ago and that it lasted for about 55 million years.

Explanation:

2. What information did Watson and Crick use for their model ?​

Answers

Ans: Crick and Watson used their findings in their own research. In April 1953, they published the news of their discovery, a molecular structure of DNA based on all its known features - the double helix. Their model served to explain how DNA replicates and how hereditary information is coded on it.

When the partial pressure of O2 in venous blood is 30 Torr, the saturation of myoglobin with O2 is _______, while the saturation of hemoglobin with O2 is ______.

Answers

Answer:

0.91, 0.55

Explanation:

When the partial pressure of O2 in venous blood is 30 Torr, the saturation of myoglobin with O2 is 0.91 while the saturation of hemoglobin with O2 is 0.55

Many signaling pathways have multiple transducer proteins, for example in a multi-enzyme phosphorylation cascade - one of the purposes for this is: _________.
a. to prevent damage to the receptor protein
b. to amplify the signal
c. all choices are correct
d. to avoid gene duplication
e. to conserve ATP and/or GTP

Answers

Answer:

b. to amplify the signal

Explanation:

A multi-enzyme phosphorylation cascade is a series of signaling events where one enzyme phosphorylates to another, then this last enzyme acts to phosphorylate another protein and so successively, thereby triggering a chain reaction that leads to the phosphorylation of hundreds or even thousands of proteins. A multi-enzyme phosphorylation cascade is known to increase the number of activated (phosphorylated) proteins at each step of the signaling cascade. Phosphorylation is a posttranslational modification capable of activating proteins during long periods, thereby a phosphorylation cascade also enables the activation of multiple proteins before these proteins become inactive again.

One of the purposes of a multi-enzyme phosphorylation cascade is signal amplification, as shown in option B.

We can arrive at this answer because:

A multi-enzyme phosphorylation cascade allows the emission of a sequence of signals, which occur simultaneously.These signals are created from a series of enzymes, each of which emits a signal by phosphorylating a protein.This all promotes thousands of phosphorylations to be promoted at the same time, all giving off signals.This simultaneous emission of signals extends the range of these signals and allows the phosphorylated proteins to emit more signals, further expanding their reception.

In this case, we can state that the multi-enzyme phosphorylation cascade has the function of amplifying an emitted signal.

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If the original strand reads ATT-CCC-GCA what is the complementary strand?

Answers

Explanation:

Problem Set 4 Answers

1a. The template DNA strand, from which the mRNA is synthesized, is 5’ CAAACTACCCTGGGTTGCCAT 3’

(RNA synthesis proceeds in a 5’ à 3’ direction, so the template strand and the mRNA will be complementary to each other)

b. The coding DNA strand, which is complementary to the template strand, is 5’ ATGGCAACCCAGGGTAGTTTG 3’

c. The sequence of the mRNA is 5’ AUGGCAACCCAGGGUAGUUUG 3’

(the sequence of the mRNA is complementary to the template strand and identical to the coding strand with U substituted for T)

d. The third codon is 5’ ACC 3’. Therefore, the corresponding anti-codon is 5’ GGU 3’

2. Below is a table for the genetic code:

T

C

A

G

T

TTT Phe (F)

TTC "

TTA Leu (L)

TTG "

TCT Ser (S)

TCC "

TCA "

TCG "

TAT Tyr (Y)

TAC "

TAA Stop

TAG Stop

TGT Cys (C)

TGC "

TGA Stop

TGG Trp (W)

C

CTT Leu (L)

CTC "

CTA "

CTG "

CCT Pro (P)

CCC "

CCA "

CCG "

CAT His (H)

CAC "

CAA Gln (Q)

CAG "

CGT Arg (R)

CGC "

CGA "

CGG "

A

ATT Ile (I)

ATC "

ATA "

ATG Met (M)

ACT Thr (T)

ACC "

ACA "

ACG "

AAT Asn (N)

AAC "

AAA Lys (K)

AAG "

AGT Ser (S)

AGC "

AGA Arg (R)

AGG "

G

GTT Val (V)

GTC "

GTA "

GTG "

GCT Ala (A)

GCC "

GCA "

GCG "

GAT Asp (D)

GAC "

GAA Glu (E)

GAG "

GGT Gly (G)

GGC "

GGA "

GGG "

a. The following codons can be mutated by one base to produce an amber codon:

CAG Gln

AAG Lys

GAG Glu

TCG Ser

TTG Leu

TGG Trp

TAA Stop

TAT Tyr

TAC Tyr

The complementary strand will read TAA-GGG-CGT since the A is complementary to T and C is to G nucleotide.

What are nucleotides?

A biological molecule known as a nucleotide has the basic building blocks of a nitrogenous base, pentose sugar, and phosphate. As polynucleotides, DNA and RNA are composed of a chain of monomers with various nitrogenous bases. The execution of metabolic and physiological processes requires nucleotides.

Building blocks of nucleic acids, energy storage, carriers of activated metabolites for biosynthesis, structural moieties of coenzymes, and metabolic regulators are all roles that nucleotides play in the physiology of animals.

Adenine and thymine and guanine and cytosine are the proper bases to couple with in DNA. Therefore, the complementary strand will read TAA-GGG-CGT since the A is complementary to T and C is to G nucleotide.

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