Cell biologists studying the mobility of plasma membrane proteins fused mouse and human cells together, creating a heterokaryon. They then examined the localization of mouse and human proteins over time. Initially the mouse and human proteins were restricted to separate halves of the heterokaryon. Over time the mouse and human proteins mixed, with each protein eventually being present over the entire cell surface, consistent with proteins being able to move within the plane of the membrane. What techniques could have been used to examine the locations of the mouse and human membrane proteins

Answers

Answer 1

Answer: Fluorescence microscopy

Explanation:

A protein is substance that is the essential constituent of living cells because it forms part of its structure. They are also found in plasma membrane, where they assist substances to cross the membrane.  Proteins can be:

Integral: permanently attached within the plasma membrane.Transmembrane: it spans the entirety of the cell membrane.Peripheral: are only temporarily associated to the membrane.

Some integral proteins can act as cellular receptors. Other proteins are responsible for cell adhesion (binding of a cell to another cell or to a surface). On the outside of the cell membranes, attached to other proteins, are the carbohydrate chains that act as labels identifying the type of cell.

A heterokaryon is a multinucleate cell and in this experiment the scientists fuse a human cell and a mouse cell, each of them will have their own proteins. At first, the human and mouse proteins where found in separated halves of this heterokaryon. But after a while, those proteins where mixed and could no longer be identified.

A fluorescent chemical called a fluorophore is able to be absorb light of specific wavelengths and then emit light of longer wavelengths. The proteins can be modified and marked with different colored fluorophores to detect them, even if they are mixed in the heterokaryon. Then, they can be seen with a Fluorescence microscopy, to identify them through different colors.  


Related Questions

Which of the following statements is subjective?
A. Van Gogh painted beautiful works of art.
B. Einstein was a scientist.
C. The planets revolve around the sun.
D. Blue is a color.

The answer is

Answers

i believe the answer is a because the rest are all facts. subjectivity can vary from person to person therefore, proving that a is correct.
I think the answer is A

why is blood pressure an example of negative feedback?

Answers

Answer:

Regulation of blood pressure is an example of negative feedback. ... If blood pressure is too high, the heart rate decreases as the blood vessels increase in diameter ( vasodilation ), while the kidneys retain less water. These changes would cause the blood pressure to return to its normal range.

Explanation:

Please select the word in parentheses that fits the sentence:
A catalyst [weakens/strengthens] the bonds of the reactants [increasing/decreasing] the activation energy.

Answers

Answer:

Weakens and Lowers activation energy

Explanation:

water molecules are polar, with the​

Answers

Answer:

Water molecules are polar, with the oxygen side being slightly negative and the hydrogen side being slightly positive. ... In a water molecule, shared electrons spend more time around the oxygen atom than the hydrogen atoms. As a result, the oxygen atom is slightly negative.

Explanation:

Describe how bacteria have become resistant to treatment. ​

Answers

Answer:

Explanation:

Bacteria gene will mutate and this trait will be passed along to the offspring. This allows them to withstand treatment.

Both a man and a woman are heterozygous for freckles. Freckles (F) are completely dominant over no freckles (f). What is the chance that their child will have freckles? Show the genotypes crossed, and the probability of each genotype and phenotype that may result from the cross.

Answers

Answer:

The chances that their child will have freckles equals 75% or 0.75.  

Explanation:

Due to technical problems, I was not able to complete the explanation in this box. You will find it in the attached files.  

What is the driving force for plate tectonics?


A.Gravity slab pull
B.The gravity of the Moon and Sun
C.Heat from the Earth's core causing mantle convection

Answers

Answer:

Option C

Explanation:

Driving force for plate tectonics is the "convection in the Earths mantle" or option C. Convection is the transfer of heat due to the movement in molecules also causing hot to rise and cold to sink. Convection currents cause the tectonic plates to move because the currents rise to the Earths surface forcing the plates to drive away from each other also known as seafloor spreading.

Hope this helps

Which of the following statements is true regarding the vice president's role in the Senate?
In recent years, vice presidents have been less active in the Senate.
Most modern vice presidents have voted hundreds of times in the Senate.
Vice presidents will often get involved in debates being held in the Senate.
The vice president may cast a vote in the Senate whenever they chose.

Answers

Answer:

answer ...In Recent year, vice presidempnt have been less active in the Senate

Explanation:

hope It will help

find the solution to the equation
[tex] {x}^{2x} - 5x {}^{2} + 4 = 0 \\ [/tex]

Answers

Here you go, hope it helps

What do these panels do?

Answers

They transfer the energy from the sone into electricity

Cell division occurs in two stages: division of the nucleus, called
Choose...
, and division of the cytoplasm, called
Choose...
.

Answers

Answer:

mitosis, cytokinesis

Explanation:

Cell division occurs in two stages: the division of the nucleus, called karyokinesis, and the division of the cytoplasm, called cytokinesis, and both of these include cell division and form two cells from one.

What is the significance of karyokinesis?

There are many steps in cell division, two of which are important, the first is the division of the nucleus, known as karyokinesis, which is the process of nuclear division that includes replication of the genetic material and separation of the duplicated chromosomes and is important for the production of genetically identical daughter cells for growth and repair in multicellular organisms. The single cell is divided into two cells after cytokinesis, which occurs at the end of cell division.

Hence, cell division occurs in two stages: the division of the nucleus, called karyokinesis, and the division of the cytoplasm, called cytokinesis, and both of these include cell division and form two cells from one.

Learn more about the Karyokinesis here.

https://brainly.com/question/28196816

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Which statement explains the role of genes in the process of inheriting a specific trait? Question 8 options: Genes are changed by the environment of a living thing and these changes effect the genes of the offspring. Genes in living things come together to make chromosomes which take information from offspring to parent. Genes absorb chemicals from the blood stream and carry them throughout the body of parents and offspring. Genes which are past from both parents carry the genetic material that determines inherited traits.

Answers

Answer:

Genes which are past from both parents carry the genetic material that determines inherited traits.

Explanation:

Rotenone, a piscicide (fish poison), interferes with the transfer of electrons between Complex I and Complex II in the process shown above. Which of the following results would be expected in the mitochondria of fish exposed to Rotenone? Why?

Answers

Answer:

Mitochondria stops working.

Explanation:

When the mitochondria of fish exposed to Rotenone, it inhibits the cellular respiration which is the main cause of death of fishes. This inhibiting of cellular respiration in mitochondria leads to reduction in cellular uptake of oxygen and this reduction is responsible for the death of fishes. This Rotenone releases by the plants present in the water as well as used as pesticides for killing of fishes.

What are atoms? What particles are they composed of? What is relative location and charge of each particle?

Answers

Explanation:

an atom is the smallest chemical particle that exists

the are composed of 3 main things

protons

neutrons

electrons

protons and neutrons are located in the nucleus of the atomic structure( centre) and electrons are in shells out of the nucleus

protons are positive charged

electrons are negative charged

neutrons don't have a charge as their charge is too small and considered as nothing so 0

please help me, i dont like this

Answers

Answer:

the answer is true because plant can be infected

by parasitic

sorry if wrong

Answer:

This is true.

Explanation:

Some plants are parasitic, which means they do not make their own food. They do, however, steal necessary water and minerals from other plants, often killing the other plants off. Parasitic plants sometimes do have chlorophyll, and others do not.

Which sequence of RNA transcribed from the base sequence of DNA is ATA CCG ATC GAT

a. UAU GGC UAG CUA c. GCG CCG ATG GUA
b. TCG CCC AUG CUA d. UAU CCG AUG CUA

Answers

Answer:

a

Explanation:

this image shows what each letter changes to.

Answer:

The transcription of the sequence ATA CCG ATC GAT is a. UAU GGC UAG CUA

Explanation:

T --> A

A --> U

C --> G

G --> C

Which of the following is an example of an ecosystem service that would provide protection from a hurricane storm surge?

A lock and dam system

An intact coastal wetland

An energy-producing tidal barrage system

An offshore, deep-water fish farm

Answers

Answer: An intact coastal wetland

Explanation:

Hurricane storm surges results when the water level rises above normal as a result of a storm and therefore goes inland from the coast far deeper than it normally does.

Normally this can destroy things next to the coast by washing them away but some ecosystem services can provide protection against this such as an intact coastal wetland. Being intact, there are less chances of being washed away and as it is a wetland it is used to high levels of water and can even absorb some of the surge.

A student observed a palisade cell using a microscope. The microscope had four objective lenses, each with a different magnification. Which objective lense should the student use?

Answers

Answer:

x4 objective

Explanation:

The student should use the x4 objective lense in order to be able to observe the palisade cell of a leaf.

The palisade cells are located below the cuticle and the epidermis of the leaf and the x4 objective will provide the largest field of view for adequate study by the student. The x4 objective is also easier to focus compared to the lower objectives.

how can mass help identify an unknown substance

Answers

You can identify an unknown substance by measuring its density and comparing your result to a list of known densities. Density = mass/volume

Answer:

Explanation:

You cannot use mass to tell one substance from another.

two very different objects can have the same mass.

High metamorphism ( what is the metamorphic rock) ?

Answers

Answer:

I hope this helps you!

Explanation:

Metamorphic rocks started out as some other type of rock, but have been substantially changed from their original igneous, sedimentary, or earlier metamorphic form. Metamorphic rocks form when rocks are subjected to high heat, high pressure, hot mineral-rich fluids or, more commonly, some combination of these factors

Answer:

Metamorphism rock is...

Explanation:

Rock that was once one form of rock but has changed to another under the influence of heat, pressure, or some other agent without passing through a liquid phase.

PLEASE PLS PLS ANSWER FASTT
Which of the following is not an example of chemical weathering?

a Animals burrowing in the ground and causing rocks to become exposed and then further weathered by wind and other elements.

b Acid rain reacting with rocks.

c Plants that produce acid and slowly wear down the rocks

d Rocks that contain iron react with oxygen and water and cause rocks to turn a reddish color.

Answers

Since chemical weathering is the reaction of rocks with other chemicals, the answer should be answer D!

Hope this helps, if not, comment below please!!!

You can give them the Brainlyest

please hellp asappppp In an area with very tall trees shading an understory of shorter trees, all of which have epiphytes growing on them, which of the following other conditions are most likely to exist as well?

It is near 30° latitude north or south or on the leeward side of a mountain range.
Moist air is rising over the area.
Night temperatures are much colder than daytime temperatures.
The soil has a thick layer of decomposed, nutrient-rich organic material.
There is an extremely high diversity of species in the area.
There is little rainfall and frequent fires regularly cut the vegetation to the ground.
4 and 6
1 and 3
2 and 5
3 and 4
please please give me the brainlyest answer

Answers

I think it’s this one(There is little rainfall, and frequent fires regularly cut the vegetation to the ground.)

VERY EASY 7TH GRADE SCIENCE QUESTION FIRST PERSON BRAINLIEST!!!!

The final velocity minus the initial velocity divided by time is the formula for —

A. constant speed
B. ending velocity
C. average speed
D. acceleration

Answers

D acceleration is the answer

1.) If a strand of DNA has 10% G, what percent will be T?

2.) If a strand of DNA has 45% A, what percent will be C?

3.) If a strand of DNA has 24% T, what percent will be G?

Answers

3 is the correct answer
1. 40% T
2. 5% C
3. 26% G

Predict the two possible outcomes if temperature or pH were changed within the molecular control system of cell division.

I'm pretty sure it's that the cells would divide more or divide less, right? Because the temp or pH change would cause some enzymes to denature, and then, depending on which ones denature, the regulation of cell division would be messed up and they would either divide too much or not enough?

Answers

Answer:

Yes, pH and temperature affects the cell division.

Explanation:

If the temperature falls between the range of 3 °C to 25 °C, the mitosis process increases but when the temperature lowers from 3 °C and exceeds from 25 °C so it decreases the rate of mitosis process because the enzymes which is responsible for the process of mitosis denatures and does not work properly. same is the case with pH, the rate of the process will increase when the pH is neutral but decreases when the pH goes lower or higher.

Which of the following statements is true about the movement of fluid in a
convection cell?
A. Hot fluid rises because it is denser.
B. Cool fluid rises because it is less dense.
C. Cool fluid sinks because it is denser.
D. Hot fluid sinks because it is less dense.

Answers

The option that is true about the movement of fluid in a convection cell is B. Cool fluid rises because it is less dense.

What is a convection cell?

It should be noted that a convection cell simply means a system where a fluid is warmed and loses density.

In this case, cool fluid rises because it is less dense. The colder and denser part of the fluid simply descends below the warmer fluid.

Learn more about cells on:

https://brainly.com/question/13123319

16 An organism with a cell wall would most likely be unable to take in materials through
A) osmosis
B) facilitated diffusion
C) active transport
D) endocytosis​

Answers

Answer:

endocytocis

Explanation:

I am pretty sure that is B but it can also be D

I need help in this I’m confused

Answers

Answer:

A

Explanation:

see, if the beetle population decrease. the green plants will have nothing that eats them so they will increase. but since the owl's existence depends on every other organism to the left, any decrease will affect is negatively.

What are some of our key mineral resources and what are our known reserves?

Answers

Answer: heyy

nnejdew

Explanation:

As the fruit develops, why do the petals, stamen, and sepals fall off?
A. The fruit absorbs them, which gives the fruit its color.
B. They have served their purpose and are no longer needed by the plant.
C. The material in the petals, stamen, and sepals become the skin of the fruit.
D. The flower has become dehydrated.

Answers

Answer:

A. The fruit absorb them, which gives the fruit its color

Explanation:

basta yunn

A, the fruit absorbs them.
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